Legumain (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)

The Legumain (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary) is a highly sensitive immunoassay designed to detect Legumain, a lysosomal protease and host cell protein (HCP) produced by CHO cells during biotherapeutic manufacturing. Legumain is released from both dead and viable CHO cells into the extracellular space and can compromise the integrity, stability, and efficacy of therapeutic monoclonal antibodies by cleaving asparaginyl and aspartyl bonds.

• Target: Legumain
• Species Reactivity: Hamster (CHO)
• Detection Range: 20 ng/mL – 0.3125 ng/mL
• Sensitivity: 0.131 ng/mL
• Assay Time: ~170 minutes
• Sample Type: Tissue culture fluids
• Storage: 2–8°C
• Shelf Life: 1 year
• Application: ELISA

This kit is essential for HCP monitoring, apoptosis research, and cancer biology, helping ensure the safety and quality of CHO-derived biologics.