Pre-formatted enzyme-linked immunosorbent assay (ELISA) kits can provide significant savings in terms of time and money compared to developing and optimizing an ELISA in-house. They are especially valuable for projects that depend on consistent assay performance and reliable long-term supply, which span many different research and in vitro diagnostics (IVD) applications. Veterinary ELISA kits are used for detecting and quantifying target analytes in animal samples such as plasma, serum, or urine, where they can confirm the presence of an infectious agent or help to diagnose a disease. They are also used for analyzing tissue culture supernatants (e.g., those produced during drug testing against animal cell lines) and tissue homogenates (e.g., the various sample types generated during pre-clinical animal studies).
Veterinary ELISA kits from ICL
We manufacture a broad range of veterinary ELISA kits from our own raw materials, with all production taking place right here in the USA. This allows us to retain complete control over our products to safeguard quality and consistency, as well as provides the flexibility to scale up on demand for timely delivery on our customers’ requirements. Our ELISA kits have been widely cited in the literature across multiple fields of research, not least due to our rigorous process control and testing, which is backed by rapid customer service and support.
Our veterinary ELISA kits employ a sandwich assay format for enhanced specificity and sensitivity. They include a microplate coated with analyte-specific antibodies that are used for target capture and typically employ a second, analyte-specific antibody conjugated to horseradish peroxidase (HRP) for detection. Incubation with the chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB), allows the concentration of bound analyte to be determined against the standard, which is also included in the kit.
Maximizing the performance of our veterinary ELISA kits begins with identifying the best matched antibody pairs. This involves confirming that the two analyte-specific antibodies used for capture and detection each recognize different epitopes, which prevents competition for analyte binding, and checking that they do not cross-react with each other, which could yield unwanted background signal. We also thoroughly optimize our assay protocols, which are detailed in the product insert accompanying each kit.