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Rat CRP ELISA Kit
Catalog #
E-25CRP
Product Size
1.0
plate
The principle of the double antibody sandwich Rat CRP ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-Rat CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Rat CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of rat CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Reactivity | Rat |
| Host | Chicken |
| Specificity/Target | CRP |
| Size | 1.0 |
| Detection Range | 6.25 ng/ml - 200 ng/ml |
| Sensitivity | 1.266 ng/ml |
| Assay Time | 25 min. |
| Sample Type | Plasma, Serum, Urine |
| Storage | 2-8C |
Data Sheet
SDS
Rat CRP ELISA Kit - For the quantitative determination of c-reactive protein (CRP) in rat samples.
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