Cathepsin L (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)

The Cathepsin L (HCP) ELISA Kit – CHO is a sensitive two‑site immunoassay for detecting Cathepsin L, a lysosomal cysteine protease involved in intracellular protein degradation, antigen processing, and turnover. As a host cell protein released during CHO culture, CTSL can impact the quality and stability of biologics if not effectively removed during purification. Its potent cysteine protease activity and broad substrate range make reliable detection essential for robust HCP monitoring.

• Target: Cathepsin L (CTSD)
• Species Reactivity: Hamster (CHO)
• Detection Range: 20 ng/mL – 0.3125 ng/mL
• Sensitivity:
• Assay Time: ~170 minutes
• Sample Type: Tissue culture fluids
• Storage: 2–8°C
• Shelf Life: 1 year
• Application: ELISA

Residual Cathepsin L is considered a significant Host Cell Protein (HCP) impurity due to its strong proteolytic activity. Even trace levels of active Cathepsin L can fragment or degrade therapeutic protein products, threatening product quality, structural integrity, and ultimately patient safety.