Cathepsin D (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)

The Cathepsin D (HCP) ELISA Kit – CHO is a sensitive two‑site immunoassay for detecting Cathepsin D, a lysosomal aspartic protease involved in intracellular protein processing and turnover. As a host cell protein released during CHO culture, CTSD can affect the quality and stability of biologics if not efficiently removed. Its distinct aspartic protease activity and broad substrate specificity make reliable detection essential for robust HCP monitoring.

• Target: Cathepsin D (CTSD)
• Species Reactivity: Hamster (CHO)
• Detection Range: 20 ng/mL – 0.3125 ng/mL
• Sensitivity:
• Assay Time: ~170 minutes
• Sample Type: Tissue culture fluids
• Storage: 2–8°C
• Shelf Life: 1 year
• Application: ELISA

Residual Cathepsin D is considered a critical Host Cell Protein (HCP) impurity due to its potent aspartic protease activity. Even low levels of active Cathepsin D can cleave or destabilize therapeutic protein products, posing risks to product quality, functional integrity, and ultimately patient safety.