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Rat Albumin ELISA Kit
Catalog #
E-25AL
Product Size
1.0
plate
The principle of the double antibody sandwich Rat Albumin ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ALB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Goat |
| Target Species | Rat |
| Specificity/Target | Albumin |
| Size | 1.0 |
| Detection Range | 6.25 ng/ml - 400 ng/ml |
| Sensitivity | 1.620 ng/ml |
| Assay Time | 70 min. |
| Sample Type | Plasma, Serum, Urine |
| Storage | 2-8C |
Data Sheet
SDS
Product Citations referencing ICL's Rat Albumin ELISA Kit:
T.J. Lieberthal, T. Sahakyants, N.R. Szabo-Wexler, M.J. Hancock, A.P. Spann, M.S. Oliver, S.C. Grindy, C.M. Neville, J.P. Vacanti, Implantable 3D printed hydrogels with intrinsic channels for liver tissue engineering, Proc. Natl. Acad. Sci. U.S.A. 121 (47) e2403322121, https://doi.org/10.1073/pnas.2403322121 (2024).
Jerome JR, Deliyanti D, Suphapimol V, Kolkhof P, Wilkinson-Berka JL. Finerenone, a Non-Steroidal Mineralocorticoid Receptor Antagonist, Reduces Vascular Injury and Increases Regulatory T-Cells: Studies in Rodents with Diabetic and Neovascular Retinopathy. Int J Mol Sci. 2023;24(3):2334. Published 2023 Jan 25. doi:10.3390/ijms24032334
Hattori H et al. Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability Exp Ther Med. 2017 Mar; 13(3): 1176–1180.
Sun, Chao et al. Hepatic Differentiation of Rat Induced Pluripotent Stem Cells in Vitro. World Journal of Gastroenterology : WJG 21.39 (2015): 11118–11126. PMC. Web. 19 Dec. 2017.
Frankowski J et al. Detrimental role of the EP1 prostanoid receptor in blood-brain barrier damage following experimental ischemic stroke. Sci Rep. 2015 Dec 9;5:17956.
Waski A.A. et al. Ezrin is down-regulated in diabetic kidney glomeruli and regulates actin reorganization and glucose uptake via GLUT1 in cultured podocytes (2014) American Journal of Pathology, 184 (6) , pp. 1727-1739. DOI: http://dx.doi.org/10.1016/j.ajpath.2014.03.002
Terawaki K et al. New cancer cachexia rat model generated by implantation of a peritoneal dissemination-derived human stomach cancer cell line. Articles in PresS. Am J Physiol Endocrinol Metab (December 17, 2013). doi:10.1152/ajpendo.00116.2013
Thomsen KL, Jessen N, Møller AB, et al. Regulation of urea synthesis during the acute-phase response in rats. Am J Physiol Gastrointest Liver Physiol. 2013;304(7):G680-G686. doi:10.1152/ajpgi.00416.2012
Gawrys O et al., Effects of liposomes with polyisoprenoids, potential drug carriers,on the cardiovascular and excretory system in rats. Pharmacological Reports Volume 66, Issue 2, April 2014, Pages 273–278
Bhoopalan V et al. Tobacco smoke modulates ozone-induced toxicity in rat lungs and central nervous system Inhalation Toxicology Vol. 25, Iss. 1, 2013 Pages 21-28
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