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Mouse SAA ELISA Kit
Catalog #
E-90SAA
Product Size
1.0
plate
The principle of the double antibody sandwich Mouse SAA (serum amyloid a) ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-Mouse SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Goat |
| Target Species | Mouse |
| Specificity/Target | Serum Amyloid A |
| Size | 1.0 |
| Detection Range | 31.25 ng/ml - 2000 ng/ml |
| Sensitivity | 11.015 ng/ml |
| Assay Time | 100 min. |
| Sample Type | Plasma, Serum |
| Storage | 2-8C |
Data Sheet
SDS
Publications that include ICL's Mouse SAA ELISA Kit -
Zangara MT, Ponti AK, Miller ND, et al. Maltodextrin Consumption Impairs the Intestinal Mucus Barrier and Accelerates Colitis Through Direct Actions on the Epithelium. Front Immunol. 2022;13:841188. Published 2022 Mar 14. doi:10.3389/fimmu.2022.841188
Hong KB, Kim D, Kim BK, et al. CF₃-Substituted Mollugin 2-(4-Morpholinyl)-ethyl ester as a Potential Anti-inflammatory Agent with Improved Aqueous Solubility and Metabolic Stability. Molecules. 2018;23(8):2030. Published 2018 Aug 14. doi:10.3390/molecules23082030
Yoon H, Schaubeck M, Lagkouvardos I, et al. Increased Pancreatic Protease Activity in Response to Antibiotics Impairs Gut Barrier and Triggers Colitis. Cell Mol Gastroenterol Hepatol. 2018;6(3):370-388.e3. Published 2018 May 29. doi:10.1016/j.jcmgh.2018.05.008
Ménoret A et al. Trace Levels of Staphylococcal Enterotoxin Bioactivity Are Concealed in a Mucosal Niche during Pulmonary Inflammation. (2015) PLoS ONE 10(10): e0141548. doi:10.1371/journal.pone.0141548.
Brancaleone et al. A vasculo-protective circuit centered on lipoxin A4 and aspirin-triggered 15-epi-lipoxin A4 operative in murine microcirculation Blood: 122 (4) Pages: 608 - 617 DOI: http://dx.doi.org/10.1182/blood-2013-04-496661
Girard TJ, Lasky NM, Tuley EA, Broze GJ Jr. Protein Z, protein Z-dependent protease inhibitor (serpinA10), and the acute-phase response. J Thromb Haemost. 2013;11(2):375-378. doi:10.1111/jth.12084
Quinton LJ, Jones MR, Robson BE, Mizgerd JP. Mechanisms of the hepatic acute-phase response during bacterial pneumonia. Infect Immun. 2009;77(6):2417-2426. doi:10.1128/IAI.01300-08
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