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Mouse IgE ELISA Kit
Catalog #
E-90E
Product Size
1.0
plate
The assay is a double antibody sandwich Mouse IgE ELISA. In this assay the IgE present in samples reacts with the anti-Mouse IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Goat |
| Target Species | Mouse |
| Specificity/Target | IgE |
| Size | 1.0 |
| Detection Range | 6.25 ng/ml - 400 ng/ml |
| Sensitivity | 1.911 ng/ml |
| Assay Time | 70 min. |
| Sample Type | Plasma, Serum |
| Storage | 2-8C |
Data Sheet
SDS
Product Citations:
Choi M, Lee JH, Lee YJ, Paik HD, Park E. Egg Yolk Protein Water Extracts Modulate the Immune Response in BALB/c Mice with Immune Dysfunction Caused by Forced Swimming. Foods. 2022;11(1):121. Published 2022 Jan 4. doi:10.3390/foods11010121
Nagpal PS, Kesarwani A, Sahu P, Upadhyay P. Aerosol immunization by alginate coated mycobacterium (BCG/MIP) particles provide enhanced immune response and protective efficacy than aerosol of plain mycobacterium against M.tb. H37Rv infection in mice. BMC Infect Dis. 2019;19(1):568. Published 2019 Jul 1. doi:10.1186/s12879-019-4157-2
Yang J, et al. Induction of Regulatory Dendritic Cells by Lactobacillus paracasei L9 Prevents Allergic Sensitization to Bovine β-Lactoglobulin in MiceS Journal of Microbiology and Biotechnology. 2015. Oct, 25(10): 1687-1696
Wang JH, Fan SW, Zhu WY. Development of Gut Microbiota in a Mouse Model of Ovalbumin-induced Allergic Diarrhea under Sub-barrier System. Asian-Australas J Anim Sci. 2013;26(4):545-551. doi:10.5713/ajas.2012.12502
Yockey LJ et al. The absence of a microbiota enhances TSLP expression in mice with defective skin barrier but does not affect the severity of their allergic inflammation. The Journal of investigative dermatology. 2013;133(12):2714-2721. doi:10.1038/jid.2013.228.
Duan J, Sugawara T, Sakai S, Aida K, Hirata T. Oral glucosylceramide reduces 2,4-dinitrofluorobenzene induced inflammatory response in mice by reducing TNF-alpha levels and leukocyte infiltration. Lipids. 2011;46(6):505-512. doi:10.1007/s11745-010-3518-9
LV PP et al. Porous quaternized chitosan nanoparticles containing paclitaxel nanocrystals improved therapeutic efficacy in non-small-cell lung cancer after oral administration Biomacromolecules. 2011 Dec 12;12(12):4230-9. doi: 10.1021/bm2010774. Epub 2011 Nov 10.
Yibu Kong, Aiai Dong, Xinyan Yu, Ting Sun, Hongjun Yu, Chengxiang Zhang, Chunyu Li, Yongji Wang, Yinan Guo,
YiQi GuBen formula targets EHMT2 and enhances NEDD4L transcription to combat epithelial-mesenchymal transition and airway remodeling in asthma, Journal of Ethnopharmacology, Volume 359, 2026, 121125, ISSN 0378-8741,
https://doi.org/10.1016/j.jep.2025.121125.
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