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Dog CRP ELISA Kit
Catalog #
E-40CRP
Product Size
1.0
plate
C-reactive protein (CRP) is a substance produced by the liver in response to inflammation. It is a marker of inflammation in the body and is often used in medical settings to assess the presence and severity of inflammation. CRP levels can rise in response to various conditions, including infections, injuries, and chronic inflammatory diseases.
High levels of CRP in the blood can indicate the presence of inflammation, but it doesn't pinpoint the exact cause. It is commonly used in combination with other clinical and laboratory findings to help diagnose and monitor conditions such as infections, autoimmune diseases, and cardiovascular diseases. Elevated CRP levels may also be associated with conditions like rheumatoid arthritis, inflammatory bowel disease, and certain cancers.
The principle of the double antibody sandwich Dog/Canine CRP ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-Dog CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Dog CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of canine CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Chicken |
| Target Species | Dog/Canine |
| Specificity/Target | CRP |
| Size | 1.0 |
| Detection Range | 3.125 ng/ml - 200 ng/ml |
| Sensitivity | 0.855 ng/ml |
| Assay Time | 25 min. |
| Sample Type | Plasma, Serum, Urine |
| Storage | 2-8C |
Data Sheet
SDS
Publications citing the use of ICL's Dog CRP ELISA kit -
DeClue A. et al. Identification of Immunologic and Clinical Characteristics That Predict Inflammatory Response to C. Novyi-NT Bacteriolytic Immunotherapy. BMC Veterinary Research 14 (2018): 119. PMC. Web. 9 July 2018.
Liu DJX, Meyer E, Broeckx BJG, et al. Variability of serum concentrations of cystatin C and urinary retinol-binding protein, neutrophil gelatinase-associated lipocalin, immunoglobulin G, and C-reactive protein in dogs. J Vet Intern Med. 2018;32(5):1659-1664. doi:10.1111/jvim.15293
Sample S. et al. Use of a platelet-rich plasma-collagen scaffold as a bioenhanced repair treatment for management of partial cruciate ligament rupture in dogs. PLoS ONE 13(6): e0197204. https://doi.org/10.1371/journal.pone.0197204
Sample S. et al. Radiographic and Magnetic Resonance Imaging Predicts Severity of Cruciate Ligament Fiber Damage and Synovitis in Dogs with Cranial Cruciate Ligament Rupture. Ed. Douglas Thamm. PLoS ONE 12.6 (2017): e0178086. PMC. Web. 9 July 2018.
Defauw P, Meyer E, Duchateau L, Schoeman JP, Van de Maele I, Daminet S. Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage. J Vet Diagn Invest. 2017;29(3):346-350. doi:10.1177/1040638717695608
Muir P. et al. Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture PLoS ONE 11(8): e0159095. (2016) https://doi.org/10.1371/journal.pone.0159095
Maddens B et al. Evaluation of Kidney Injury in Dogs with Pyometra Based on Proteinuria, Renal Histomorphology, and Urinary Biomarkers J Vet Intern Med. 2011 Sep-Oct;25(5):1075-83. doi: 10.1111/j.1939-1676.2011.0772.x. Epub 2011 Aug 16.
Smets P et al. Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease JVet Intern Med 2010;24:65–72
Schoeman J et al. Assessment of renal dysfunction using urinary markers in canine babesiosis caused by Babesia rossi Veterinary Parasitology Volume 190, Issues 3–4, 21 December 2012, Pages 326–332
Hrovat A et al. Evaluation of snake envenomation induced renal dysfunction in dogs using early urinary biomarkers of nephrotoxicity The Veterinary Journal Volume 198, Issue 1, October 2013, Pages 239–244
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