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Dog Albumin ELISA Kit
Catalog #
E-40AL
Product Size
1.0
plate
The principle of the double antibody sandwich Dog Albumin ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ALB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Chicken |
| Target Species | Dog/Canine |
| Specificity/Target | Albumin |
| Size | 1.0 |
| Detection Range | 12.5 ng/ml - 400 ng/ml |
| Sensitivity | 2.463 ng/ml |
| Assay Time | 100 min. |
| Sample Type | Plasma, Serum, Urine |
| Storage | 2-8C |
Data Sheet
SDS
Marynissen, S., Daminet, S., Meyer, E., Demeyere, K., Stock, E., Delanghe, J., Duchateau, L. and Paepe, D. (2025), Longitudinal Study of Renal Health Screening in Apparently Healthy Aging Dogs. J Vet Intern Med, 39: e70116. https://doi.org/10.1111/jvim.70116
For the quantitative determination of canine/dog albumin in biological samples. Lavoué R et al. (2015) Characterization of Proteinuria in Dogue de Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy: A Retrospective Study. PLoS ONE 10(7): e0133311. https://doi.org/10.1371/journal.pone.0133311
Smets, P., et al. (2012). Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration. PLoS ONE, 7(2), e31702. https://doi.org/10.1371/journal.pone.0031702
Maddens B, et al. Evaluation of kidney injury in dogs with pyometra based on proteinuria, renal histomorphology, and urinary biomarkers. J Vet Intern Med. 2011;25(5):1075–1083. doi:10.1111/j.1939-1676.2011.0756.x
Hrovat A, et al. Evaluation of snake envenomation-induced renal dysfunction in dogs using early urinary biomarkers of nephrotoxicity. Vet J. 2013;198(1):239–244. https://doi.org/10.1016/j.tvjl.2013.07.013
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