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Human Alpha 1-Antichymotrypsin ELISA Kit
Catalog #
E-80CYT
Product Size
1.0
plate
The principle of the double antibody sandwich Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA is represented in Figure 1. In this assay the Alpha 1-Antichymotrypsin present in samples reacts with the anti-Human Alpha 1-Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1-Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1-Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1-Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1-Antichymotrypsin in the test sample. The quantity of Alpha 1-Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Goat |
| Target Species | Human |
| Specificity/Target | Alpha 1-Antichymotrypsin |
| Size | 1.0 |
| Detection Range | 6.25 ng/ml - 400 ng/ml |
| Sensitivity | 2.518 ng/ml |
| Assay Time | 80 min. |
| Sample Type | Plasma, Serum |
| Storage | 2-8C |
Data Sheet
SDS
Product Citations:
Boen, H.M., Pype, L.L., Papadimitriou, K. et al. Dynamics of SERPINA3 in response to anthracycline treatment and cardiovascular dysfunction. Cardio-Oncology 11, 27 (2025). https://doi.org/10.1186/s40959-025-00324-7
Gu Y, Vorburger R, Scarmeas N, et al. Circulating inflammatory biomarkers in relation to brain structural measurements in a non-demented elderly population. Brain Behav Immun. 2017;65:150-160. doi:10.1016/j.bbi.2017.04.022
Rahman S, Rabbani R, Wachihi C, et al. Mucosal serpin A1 and A3 levels in HIV highly exposed sero-negative women are affected by the menstrual cycle and hormonal contraceptives but are independent of epidemiological confounders. Am J Reprod Immunol. 2013;69(1):64-72. doi:10.1111/aji.12014
Wågsäter D, et al. Serine protease inhibitor A3 in atherosclerosis and aneurysm disease INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 30: 288-294, 2012
Zeng X, Hood BL, Sun M, et al. Lung cancer serum biomarker discovery using glycoprotein capture and liquid chromatography mass spectrometry. J Proteome Res. 2010;9(12):6440-6449. doi:10.1021/pr100696n
Manal L NIAZI, M.D. and MAGED ABD EL-NASEER, M.D Serum Inflammatory Markers in Dementia Disorders Med. J. Cairo Univ., Vol. 77, No. 4, June: 337-346, 2009
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