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Dog IgG ELISA Kit
Catalog #
E-40G
Product Size
1.0
plate
The Dog IgG ELISA Kit from Immunology Consultants Laboratory (ICL) is a robust and reliable assay designed for the quantitative determination of total canine Immunoglobulin G (IgG) in serum and plasma samples. This double antibody sandwich ELISA utilizes goat anti-dog IgG Fc antibodies and HRP-conjugated detection antibodies to ensure high specificity and sensitivity.
The principle of the double antibody sandwich dog IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Dog IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Dog IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of dog IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolataed from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Host | Goat |
| Target Species | Dog/Canine |
| Specificity/Target | IgG h+l |
| Size | 1.0 |
| Detection Range | 25 ng/ml - 800 ng/ml |
| Sensitivity | 7.148 ng/ml |
| Assay Time | 40 min. |
| Sample Type | Plasma, Serum |
| Storage | 2-8C |
Data Sheet
SDS
Jocelyn F Wren, Sofia M Wilson, Yifei Kang, Patricia M Oba, John F Menton, Elena Vinay, Mathieu Millette, Melissa R Kelly, Kelly S Swanson, Effects of Live Bacillus pumilus SG154 or a Lacticaseibacillus paracasei 327 Postbiotic on Apparent Total Tract Nutrient Digestibility and the Immune Function and Fecal Characteristics, Metabolites, and Microbiota of Healthy Adult Dogs, Journal of Animal Science, 2025;, skaf231, https://doi.org/10.1093/jas/skaf231Product Citations:
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Alonge S. et al. Pre- and Probiotics to Increase the Immune Power of Colostrum in Dogs Front. Vet. Sci., 06 November 2020 | https://doi.org/10.3389/fvets.2020.570414
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Lavoué R et al. (2015) Characterization of Proteinuria in Dogue de Bordeaux Dogs, a Breed Predisposed to a Familial Glomerulonephropathy: A Retrospective Study. PLoS ONE 10(7): e0133311. https://doi.org/10.1371/journal.pone.0133311
Smets P et al. Renal Function and Morphology in Aged Beagle Dogs Before and after Hydrocortisone Administration. Brown JD, ed. PLoS ONE. 2012;7(2):e31702. doi:10.1371/journal.pone.0031702.
Maddens B et al. Evaluation of Kidney Injury in Dogs with Pyometra Based on Pro-teinuria, Renal Histomorphology, and Urinary Biomarkers J Vet Intern Med 2011;:1–9
Hrovat A et al. Evaluation of snake envenomation induced renal dysfunction in dogs using early urinary biomarkers of nephrotoxicity The Veterinary Journal Volume 198, Issue 1, October 2013, Pages 239–244
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