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Rat CRP ELISA Kit

CATALOG # E-25CRP
Rat CRP ELISA Kit - For the quantitative determination of c-reactive protein (CRP) in rat samples.

Rayhana B et al.
Evaluation of Anti-inflammatory Effect of Careya arboreain CFA Induced Chronic Inflammation
Int. J. Pharm. Sci. Rev. Res., 26(2), May –Jun 2014; Article No. 50, Pages: 292-298

Drafi F et al.
Pharmacological influence on processes of adjuvant arthritis: Effect of the combination of an antioxidant active substance with methotrexate.
Interdisciplinary Toxicology. 2012;5(2):84-91. doi:10.2478/v10102-012-0015-4.

Bauerova K et al
Chondroitin sulfate effect on induced arthritis in rats
Osteoarthritis and Cartilage doi:10.1016/j.joca.2011.08.006

Stofkova A et al
Effect of Feeding Status on Adjuvant Arthritis Severity, Cachexia, and Insulin Sensitivity in Male Lewis Rats
Mediators of Inflammation Volume 2010 (2010), Article ID 398026, 12 pages

Bauerova K et al.
Modern Pharmacological Approaches to Therapies: Substances Tested in Animal Models of Rheumatoid Arthritis
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences
Size 1.0 plate
Price
$375.00
OR

Details

  • Rat
  • Chicken
  • CRP
  • 4C
  • Plasma, Serum, Urine
  • 6.25 ng/ml - 200 ng/ml
  • 1.266 ng/ml
  • 25 min.
  • The principle of the double antibody sandwich Rat CRP ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-Rat CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Rat CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of rat CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

This product is for research use only, not for diagnostic or therapeutic use.