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Mouse SAA ELISA Kit

CATALOG # E-90SAA
Publications that include ICL's Mouse SAA ELISA Kit -

Ménoret A et al.
Trace Levels of Staphylococcal Enterotoxin Bioactivity Are Concealed in a Mucosal Niche during Pulmonary Inflammation.
(2015) PLoS ONE 10(10): e0141548. doi:10.1371/journal.pone.0141548.

Brancaleone et al.
A vasculo-protective circuit centered on lipoxin A4 and aspirin-triggered 15-epi-lipoxin A4 operative in murine microcirculation
Blood: 122 (4) Pages: 608 - 617 DOI: http://dx.doi.org/10.1182/blood-2013-04-496661
Size 1.0 plate
Price
$325.00
OR

Details

  • Mouse
  • Goat
  • ELISA
  • SAA
  • 4C
  • Plasma, Serum
  • 31.25 ng/ml - 2000 ng/ml
  • 11.015 ng/ml
  • 100 min.
  • The principle of the double antibody sandwich Mouse SAA (serum amyloid a) ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-Mouse SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

 

This product is for research use only, not for diagnostic or therapeutic use.