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Mouse IgG ELISA Kit

CATALOG # E-90G
Publications citing ICL's Mouse IgG ELISA Kit -

Tang M et al.
Brain microvasculature defects and Glut1 deficiency syndrome averted by early repletion of the glucose transporter-1 protein
Nature Communications 8, Article number: 14152 (2017) doi:10.1038/ncomms14152

Franz BJ et al.
Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB
Journal of Immunology Research Volume 2015 (2015), Article ID 840842, 13 pages

Iwamuro M et al.
A preliminary study for constructing a bioartificial liver device with induced pluripotent stem cell-derived hepatocytes
BioMedical Engineering OnLine 2012, 11:93 doi:10.1186/1475-925X-11-93

Santiago ML et al.
Innate Retroviral Restriction by Apobec3 Promotes Antibody Affinity Maturation In Vivo
The Journal of Immunology July 15, 2010 vol. 185 no. 2 1114-1123

Shaoa H et al.
A novel monoclonal antibody effective against lethal challenge with swine-lineage and 2009 pandemic H1N1 influenza viruses in mice
doi:10.1016/j.virol.2011.06.021




Size 1.0 plate
Price
$245.00
OR

Details

  • Mouse
  • Goat
  • IgG h+l
  • 4C*
  • Plasma, Serum
  • 9.375 ng/ml - 600 ng/ml
  • 2.084 ng/ml
  • 100 min.
  • The principle of the double antibody sandwich Mouse IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Mouse IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

This product is for research use only, not for diagnostic or therapeutic use.