Sales & Questions (503) 747-2454

Mouse IgE ELISA Kit

CATALOG # E-90E
Publications citing ICL's Mouse IgE ELISA kit:

Yang J, et al.
Induction of Regulatory Dendritic Cells by Lactobacillus paracasei L9 Prevents Allergic Sensitization to Bovine β-Lactoglobulin in MiceS
Journal of Microbiology and Biotechnology. 2015. Oct, 25(10): 1687-1696

Yockey LJ et al.
The absence of a microbiota enhances TSLP expression in mice with defective skin barrier but does not affect the severity of their allergic inflammation.
The Journal of investigative dermatology. 2013;133(12):2714-2721. doi:10.1038/jid.2013.228.

LV PP et al.
Porous quaternized chitosan nanoparticles containing paclitaxel nanocrystals improved therapeutic efficacy in non-small-cell lung cancer after oral administration
Biomacromolecules. 2011 Dec 12;12(12):4230-9. doi: 10.1021/bm2010774. Epub 2011 Nov 10.
Size 1.0 plate
Price
$285.00
OR

Details

  • Mouse
  • Goat
  • ELISA
  • IgE
  • 4C*
  • Plasma, Serum
  • 6.25 ng/ml - 400 ng/ml
  • 1.911 ng/ml
  • 70 min.
  • The assay is a double antibody sandwich Mouse IgE ELISA. In this assay the IgE present in samples reacts with the anti-Mouse IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

 

This product is for research use only, not for diagnostic or therapeutic use.