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Human Alpha 1-Antichymotrypsin ELISA Kit

Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA Kit.
This protein is synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection.

Serum Inflammatory Markers in Dementia Disorders
Med. J. Cairo Univ., Vol. 77, No. 4, June: 337-346, 2009
Size 1.0 plate


  • Human
  • Goat
  • Alpha 1-Antichymotrypsin
  • 4C
  • Plasma, Serum
  • 6.25 ng/ml - 400 ng/ml
  • 2.518 ng/ml
  • 80 min.
  • The principle of the double antibody sandwich Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA is represented in Figure 1. In this assay the Alpha 1-Antichymotrypsin present in samples reacts with the anti-Human Alpha 1-Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1-Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1-Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1-Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1-Antichymotrypsin in the test sample. The quantity of Alpha 1-Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial


This product is for research use only, not for diagnostic or therapeutic use.