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Human Albumin ELISA Kit

CATALOG # E-80AL
ICL's Human Albumin ELISA kit is intended for the quantitative determination of total human albumin in biological samples.

Lärstad M et al.
Surfactant Protein A in Exhaled Endogenous Particles Is Decreased in Chronic Obstructive Pulmonary Disease (COPD) Patients: A Pilot Study.
PLoS ONE (2015); 10(12) e0144463. doi:10.1371/journal.pone.0144463

Ericson PA et al.
Low Levels of Exhaled Surfactant Protein A Associated With BOS After Lung Transplantation
Transplant Direct. 2016 Aug 26;2(9):e103. eCollection 2016 Sep.

Halliday M et al.
Relationship Between Cyclophilin A Levels and Matrix Metalloproteinase 9 Activity in Cerebrospinal Fluid of Cognitively Normal Apolipoprotein E4 Carriers and Blood-Brain Barrier Breakdown.
JAMA Neurol. 2013;70(9):1198-1200. doi:10.1001/jamaneurol.2013.3841
Size 1.0 plate
Price
$255.00
OR

Details

  • Human
  • Goat
  • ELISA
  • Albumin
  • 4C
  • Plasma, Serum, Urine
  • 6.25 ng/ml - 200 ng/ml
  • 1.900 ng/ml
  • 100 min.
  • The principle of the double antibody sandwich Human Albumin ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ALB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

 

This product is for research use only, not for diagnostic or therapeutic use.