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Human Albumin ELISA Kit
Catalog #
E-80AL
Product Size
1.0
plate
The principle of the double antibody sandwich Human Albumin ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ALB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
| Antibody Type | ELISA |
|---|---|
| Reactivity | Human |
| Host | Goat |
| Specificity/Target | Albumin |
| Size | 1.0 |
| Detection Range | 6.25 ng/ml - 200 ng/ml |
| Sensitivity | 1.900 ng/ml |
| Assay Time | 100 min. |
| Sample Type | Plasma, Serum, Urine |
| Storage | 2-8C |
Data Sheet
SDS
Product Citations using ICL's Human Albumin ELISA Kit:
Jansson D, Wang M, Thomas RG, et al. Markers of Cerebrovascular Injury, Inflammation, and Plasma Lipids Are Associated with Alzheimer's Disease Cerebrospinal Fluid Biomarkers in Cognitively Normal Persons. J Alzheimers Dis. 2022;86(2):813-826. doi:10.3233/JAD-215400
Di Martino D, Cappelletti M, Tondo M, et al. Glycation-Driven Inflammation: COVID-19 Severity in Pregnant Women and Perinatal Outcomes. Nutrients. 2022;14(19):4037. Published 2022 Sep 28. doi:10.3390/nu14194037
Cowan, Robert P et al. Evidence that blood-CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache vol. 61,3 (2021): 536-545. doi:10.1111/head.14088
Hussain-Alkhateeb L, Bake B, Holm M, Emilsson Ö, Mirgorodskaya E, Olin AC. Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways-a European population-based cohort study. BMJ Open Respir Res. 2021;8(1):e000804. doi:10.1136/bmjresp-2020-000804
Sweeney MD, Sagare AP, Pachicano M, et al. A novel sensitive assay for detection of a biomarker of pericyte injury in cerebrospinal fluid. Alzheimers Dement. 2020;16(6):821-830. doi:10.1002/alz.12061
Piuri G, Basello K, Rossi G, et al. Methylglyoxal, Glycated Albumin, PAF, and TNF-α: Possible Inflammatory and Metabolic Biomarkers for Management of Gestational Diabetes. Nutrients. 2020;12(2):479. Published 2020 Feb 14. doi:10.3390/nu12020479
Behndig AF, Mirgorodskaya E, Blomberg A, Olin AC. Surfactant Protein A in particles in exhaled air (PExA), bronchial lavage and bronchial wash - a methodological comparison. Respir Res. 2019;20(1):214. Published 2019 Sep 26. doi:10.1186/s12931-019-1172-1
Sharma NS, Lal CV, Li JD, et al. The neutrophil chemoattractant peptide proline-glycine-proline is associated with acute respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol. 2018;315(5):L653-L661. doi:10.1152/ajplung.00308.2017
Lärstad M et al. Surfactant Protein A in Exhaled Endogenous Particles Is Decreased in Chronic Obstructive Pulmonary Disease (COPD) Patients: A Pilot Study. PLoS ONE (2015); 10(12) e0144463. doi:10.1371/journal.pone.0144463
Ericson PA et al. Low Levels of Exhaled Surfactant Protein A Associated With BOS After Lung Transplantation Transplant Direct. 2016 Aug 26;2(9):e103. eCollection 2016 Sep.
Halliday M et al. Relationship Between Cyclophilin A Levels and Matrix Metalloproteinase 9 Activity in Cerebrospinal Fluid of Cognitively Normal Apolipoprotein E4 Carriers and Blood-Brain Barrier Breakdown. JAMA Neurol. 2013;70(9):1198-1200. doi:10.1001/jamaneurol.2013.3841
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