Sales & Questions (503) 747-2454

Dog CRP ELISA Kit

CATALOG # E-40CRP
Publications citing the use of ICL's Dog CRP ELISA kit -

Muir P et al.
(2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture
PLoS ONE 11(8): e0159095. https://doi.org/10.1371/journal.pone.0159095

Maddens B et al.
Evaluation of Kidney Injury in Dogs with Pyometra Based on Proteinuria, Renal Histomorphology, and Urinary Biomarkers
J Vet Intern Med. 2011 Sep-Oct;25(5):1075-83. doi: 10.1111/j.1939-1676.2011.0772.x. Epub 2011 Aug 16.

Smets P et al.
Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease
JVet Intern Med 2010;24:65–72

Schoeman J et al.
Assessment of renal dysfunction using urinary markers in canine babesiosis caused by Babesia rossi
Veterinary Parasitology Volume 190, Issues 3–4, 21 December 2012, Pages 326–332

Hrovat A et al.
Evaluation of snake envenomation induced renal dysfunction in dogs using early urinary biomarkers of nephrotoxicity
The Veterinary Journal Volume 198, Issue 1, October 2013, Pages 239–244
Size 1.0 plate
Price
$375.00
OR

Details

  • Canine, Dog
  • Chicken
  • ELISA
  • CRP
  • 4C
  • Plasma, Serum, Urine
  • 3.125 ng/ml - 200 ng/ml
  • 0.855 ng/ml
  • 25 min.
  • The principle of the double antibody sandwich Dog/Canine CRP ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-Dog CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Dog CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of canine CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

 

This product is for research use only, not for diagnostic or therapeutic use.