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Chicken IgY ELISA Kit

CATALOG # E-30Y
The Chicken IgY ELISA kit is intended for the quantitative determination of total chicken IgY in biological samples.

Citations -

Ojiezeh TI et al.
Humoral responses of broiler chickens challenged with NDV following supplemental treatment with extracts of Aloe vera, Alma millsoni, Ganoderma lucidum and Archachatina marginata
(Cent Eur J Immunol 2015; 40 (3): 300-306) DOI: https://doi.org/10.5114/ceji.2015.54590
Size 1.0 plate
Price
$325.00
OR

Details

  • Chicken
  • Goat
  • IgY h+l
  • *
  • Plasma, Serum
  • 6.25 ng/ml - 400 ng/ml
  • 1.508 ng/ml
  • 70 min.
  • The principle of the double antibody sandwich Chicken IgY ELISA is represented in Figure 1. In this assay the IgY present in samples reacts with the anti-Chicken IgY Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Chicken IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of chicken IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

This product is for research use only, not for diagnostic or therapeutic use.