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Category : Infectious Disease Reagents :   Antibodies to Dengue Virus : Rabbit anti-Dengue (IgG Fraction)




Item Rabbit anti-Dengue (IgG Fraction) More Details :



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Host Rabbit
Catalog Number RDV-55G
Sales Price $150.00
Quantity 1.0 ml
Physical State Liquid
Buffer PBS pH 7.2
Preservative 0.1% sodium azide
Storage Frozen for long term or 4 C for short term
Procedure Rabbits were immunized with a mixture of inactivated dengue viruses of Types 1, 2, 3, and 4. Antigen was given in Freunds adjuvant. The resulting antiserum was absorbed on a fetal calf serum affinity column. An IgG fraction was prepared using Protein G affinity chromatography.
Specification The antiserum reacts with each of the dengue serotypes when tested by fluorescent antibody techniques using virus infected cells. It also reacts in dot blot and ELISA procedures. The antigens were grown in mosquito cell lines containing fetal calf serum therefore minor reactivity might be expected to antigens from these sources.
Western Blots The required dilutions must be determined for the particular assay employed.
ELISA The required dilutions must be determined for the particular assay employed.
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