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Bovine IgG ELISA Kit

CATALOG # E-10G
The Bovine IgG ELISA kit is intended for the quantitative determination of total bovine IgG in biological samples.
Size 1.0 plate
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Details

  • Bovine, Cow
  • Rabbit
  • ELISA
  • IgG h+l
  • 4C*
  • Milk, Plasma, Serum, Tissue Culture Fluids
  • 7.813 ng/ml - 500 ng/ml
  • 3.439 ng/ml
  • 50 min.
  • The principle of the double antibody sandwich Bovine IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Bovine IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Bovine IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of bovine IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Documents

Kit Contents

  • 1

    One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody

  • 2

    One ELISA Kit Data Sheet

  • 3

    One Certificate of Analysis

  • 4

    One 50 mL bottle of Diluent Running Buffer

  • 5

    One 50 mL bottle of 20X Concentrated Wash Solution

  • 6

    One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer

  • 7

    One 12 mL vial of Chromogen-Substrate Solution

  • 8

    One 12 mL vial of Stop Solution

  • 9

    One Calibrator Vial

This product is for research use only, not for diagnostic or therapeutic use.