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anti-6xHIS Tag Antibody (Rabbit) - Affinity Purified
The 6xHis tag is a commonly used protein purification tag in molecular biology and biochemistry. It consists of a short amino acid sequence containing six consecutive histidine residues (His). This tag is often genetically fused to the target protein of interest, allowing for purification of the fusion protein using affinity chromatography.
Antibody Type | Primary Antibody |
---|---|
Reactivity | 6-HIS Tag |
Host | Rabbit |
Specificity/Target | 6xHIS tag |
Size | 1.0 |
Clonality | Polyclonal |
Type | Unconjugated |
Isotype | IgG |
Origin | USA |
Shelf Life | 2 years |
Format | Liquid |
Buffer | Phospate buffered saline, pH 7.2 |
Preservative | 0.09% sodium azide |
Storage | 2-8C |
Application | ELISA |
Purity | Antigen Affinity Purified |
Product Citations referencing the use of ICL's Affinity Purified Rabbit anti-6xHIS tag Antibody:
Lu P et al. Modeling-Enabled Characterization of Novel NLRX1 Ligands. PLoS ONE 10(12): e0145420. doi:10.1371/journal.pone.0145420
Chang, How-Yi et al. “Processing Is Required for a Fully Functional Protein P30 in Mycoplasma Pneumoniae Gliding and Cytadherence .” Journal of Bacteriology 193.20 (2011): 5841–5846. PMC. Web. 10 Feb. 2018.
Kale SD, Tyler BM. Identification of lipid-binding effectors. Methods Mol Biol. 2012;835:393-414. doi:10.1007/978-1-61779-501-5_24
Relich RF et al. Insights into the function of Mycoplasma pneumoniae protein P30 from orthologous gene replacement Microbiology, October 2011 157: 2862-2870, doi: 10.1099/mic.0.052464-0
Mosnier LO et al. Hyperantithrombotic, noncytoprotective Glu149Ala-activated protein C mutant. Blood. 2009;113(23):5970-5978. doi:10.1182/blood-2008-10-183327.
Krylova I et al. Structural Analyses Reveal Phosphatidyl Inositols as Ligands for the NR5 Orphan Receptors SF-1 and LRH-1 Cell , Volume 120 , Issue 3 , 343 - 355